Journal: Microbiology Spectrum
Article Title: Cell wall nanoparticles from hyphae of Alternaria infectoria grown with caspofungin, nikkomycin, or pyroquilon trigger different activation profiles in macrophages
doi: 10.1128/spectrum.00645-24
Figure Lengend Snippet: CWNPs characterization. ( A ) Representation of a CWNP obtained from A. infectoria mycelia cell wall, with negative zeta potential (mean ± SD) and approximately 200 nm diameter. ( B ) Image of ctCWNPs obtained using TEM (scale bar = 200 nm); the images obtained were used to observe the morphology and to quantify the diameter of the CWNPs. ( C ) Nanoparticles’ zeta potential, PDI, and area analyzed using Beckman Coulter DelsaTM Nano C Particle Analyser instrument; the diameter was quantified using TEM images. ( D ) Flow cytometry signatures of the CWNPs analyzed on Partec Cyflow with CWNPs labeled with fluorescein isothiocyanate (FITC). ctCWNPs, cell wall nanoparticles prepared from mycelia of A. infectoria grown under control conditions; casCWNPs, growth medium supplemented with caspofungin 1 µg/mL; nkCWNPs with nikkomycin Z 0.5 µg/mL; or pyrCWNPs, with pyroquilon 50 µg/mL.
Article Snippet: It was performed on Partec CyFlow space instrument.
Techniques: Zeta Potential Analyzer, Flow Cytometry, Labeling, Control